Malaysian Journal of Biochemistry
& Molecular Biology
(E-ISSN: 2600-9005)
The Official Publication of the Malaysian Society for Biochemistry & Molecular Biology (MSBMB)
Indexed by SCOPUS and Malaysian Citation Index (MYCITE)
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April 2026
Malay. J. Biochem. Mol. Biol. (2026) 29 (1)
Page range 1-130
Regular Article
DOI:
Page 1- 8
Si Qi Ng, Zhen Sheng Bong, Siow-Ping Tan, Ghim Hock Ong, Khye Er Loh *
ANTI-INFLAMMATORY ACTIVITY OF GREEN SYNTHESISED SILVER NANOPARTICLES FROM Chrysanthemum morifolium EXTRACT AGAINST MSU CRYSTAL-INDUCED INFLAMMATION IN MACROPHAGE
Abstract
Gout is a form of arthritis caused by the elevated uric acid level, which is deposited as monosodium urate (MSU) crystals in the joint. Macrophages recognise the MSU crystal as a foreign antigen and trigger signalling pathways that produce pro-inflammatory cytokines (IL-1β) and inflammatory mediators (NO). Many advocates for the adoption of biological green synthesis as the primary method for nanoparticle production due to its environmental benefits, lower energy consumption, and higher yield. The properties of silver nanoparticles have been widely investigated and known to exhibit antibacterial, antimicrobial, anticancer effects and more. These effects are attributed to their high surface area-to-volume ratio at the nanoscale and multicomponent activities from its surrounding phytocompound derived from medicinal plants. Chrysanthemum morifolium (CM) is a flower that has been a big part of Chinese medicine for millennia. In this study, silver nanoparticles were synthesised using C. morifolium extract via green synthesis method. The nanoparticles produced were characterised using FTIR, UV-Vis spectroscopy and dynamic light scattering analysis. Its effect on cell viability was examined using the MTT assay, and its anti-inflammatory effects were compared to those of chemically synthesised silver nanoparticles on MSU crystal-stimulated macrophages via the Griess assay and IL-1β assay. The results revealed that the CM-AgNP synthesis was non-cytotoxic at all concentrations tested (5-25 μg/mL) while chemically synthesised AgNP showed cytotoxicity at 20 and 25 μg/mL. CM-AgNP inhibited the levels of nitric oxide and IL-1β significantly. The CM-AgNP showed promising potential as an anti- inflammatory agent and provides a safer alternative to chemically synthesised AgNPs, further study to validate its anti-inflammatory potential is suggested.