Malaysian Journal of Biochemistry
& Molecular Biology
(E-ISSN: 2600-9005)
The Official Publication of the Malaysian Society for Biochemistry & Molecular Biology (MSBMB)
Indexed by SCOPUS and Malaysian Citation Index (MYCITE)
NEW ANNOUNCEMENT
The MJBMB will be revising its publication fee for accepted papers from MYR250 to MYR300 effective 1st January 2026.
New submissions received from 1st January 2026 onwards will pay the new rate.
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Special Issue (1) 2026
Selected Papers from the:
Korea-Asean Joint Symposium on Biomass Utilisation and Renewable Energy 2024 & AFOB Regional Symposium 2025
Page range 1-67
DOI:
Page 1- 7
Indah Khairunnisa, Endang Linirin Widiastuti, Achmad Arifiyanto, Nuning Nurcahyani and Devi Ardiyati Khasana
ANTIPROLIFERATIVE EFFECTS OF ETHANOLIC EXTRATION OF SEAWEED (Padina sp.) ON HUMAN LUNG CANCER CELLS
Abstract
The coastal waters of Pesawaran, Lampung, are characterized by a high diversity and abundance of macroalgae, representing a promising source of bioactive secondary metabolites. Compounds derived from macroalgae, Padina sp, have demonstrated potential as anticancer agents. This study aimed to evaluate the cytotoxic and antiproliferative effects of these natural products against A549 human lung cancer cell lines. The experimental methods employed in this research included phytochemical screening, Fourier-transform infrared (FTIR) spectroscopy, antioxidant activity assessment using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, toxicity evaluation via the brine shrimp lethality test (BSLT), and cytotoxicity testing using the water-soluble tetrazolium (WST) assay. Phytochemical analysis revealed that the 96% ethanol extracts of Padina sp. contained different types of secondary metabolites, including alkaloids, saponins, flavonoids, phenolics, tannins, terpenoids, and steroids. The BSLT results indicated that the extract, exhibited low toxicity, with LC₅₀ values of 163.52 ppm, suggesting potential anticancer activity. Furthermore, cytotoxicity testing of the Padina sp. extract against A549 cell cultures yielded an IC₅₀ value of 336.6 µg/mL, indicating cytotoxic activity with a classification in the low toxicity category. These findings support the potential application of Padina sp extracts as complementary candidates in the development of anticancer therapies.