Special Issue (2) 2026
Selected Papers from the:

3rd International Conference on Industry-Academia Initiatives in Biotechnology and Chemistry (iCIABC2025)

Page range 1-141

DOI:

Page 37- 43

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Thevika M. Sarawanan, Rohaida Che Man, Siti Zubaidah Sulaiman, Siti Kholijah Abdul Mudalip

and Shalyda Md Shaarani

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SCREENING AND INITIAL CHARACTERIZATION OF POULTRY FEED HYDROLYSIS USING PINEAPPLE WASTE DERIVED ENZYMES

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Abstract 

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Poultry feed often contains non-starch polysaccharides (NSPs), which can cause increased intestinal viscosity and hinder nutrient absorption, negatively impacting poultry growth and feed efficiency. To reduce viscosity and improve digestibility, enzymes are often supplemented in poultry diets to breakdown the NSPs. Pineapple processing industries generate significant waste containing enzymes, providing a sustainable solution for waste valorisation. This study aimed to screen key parameters affecting poultry feed hydrolysis using enzymes derived from pineapple waste. A One Factor (RSM) design was employed to evaluate the effects of pH (5–10), incubation time (5–60 minutes) and enzyme concentration (2000–6000 mg/ml) on poultry feed hydrolysis using pineapple waste derived enzymes. Reducing sugar (Xylose) and amino acid (L-tyrosine) releases were measured using the DNS and Folin-Ciocalteu methods, respectively. The highest xylose release was observed at pH 6.5 (8.521 mg/ml), 30-minute incubation (14.854 mg/ml), and 3500 mg/ml enzyme concentration (10.198 mg/ml). For L-tyrosine, the maximum release occurred at pH 7.5 (14.250 µmol), with 30-minute incubation (12.481 µmol), and 3500 mg/ml enzyme concentration (8.328 µmol). Carbon, Hydrogen, Nitrogen and Sulphur (CHNS) analysis and Field Emission Scanning Electron Microscopy (FESEM) were used for initial characterization of feed and pineapple waste revealed that poultry feed exhibited a compact surface morphology together with high carbon and nitrogen content meanwhile pineapple waste showed a fibrous, porous surface structure and a lower carbon-nitrogen profile. These differences strongly support the use of pineapple waste-derived enzymes in poultry feed hydrolysis. Unlike many previous studies that focused only on poultry performance outcomes, this research provides mechanistic insights into enzyme-feed interactions and highlights the novel application of pineapple waste-derived enzymes for direct feed hydrolysis.

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Full text​​

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