Malaysian Journal of Biochemistry
& Molecular Biology
(E-ISSN: 2600-9005)
The Official Publication of the Malaysian Society for Biochemistry & Molecular Biology (MSBMB)
Indexed by SCOPUS and Malaysian Citation Index (MYCITE)
NEW ANNOUNCEMENT
The MJBMB will be revising its publication fee for accepted papers from MYR250 to MYR300 effective 1st January 2026.
New submissions received from 1st January 2026 onwards will pay the new rate.
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Special Issue (1) 2026
Selected Papers from the:
Korea-Asean Joint Symposium on Biomass Utilisation and Renewable Energy 2024 & AFOB Regional Symposium 2025
Page range 1-67
DOI:
Page 16-21
Syazayasmin Sabparie, Awang Ahmad Sallehin Awang Husaini, Ngieng Ngui Sing, Hairul Azman Roslan and Tay Meng Guan
ENDOPHYTIC TRICHODERMA SPP. AS BIOCONTROL AGENTS AGAINST PHYTOPHTHORA CAPSICI, PYRICULARIA ORYZAE AND FUSARIUM VERTICILLIOIDES
Abstract
Trichoderma spp. are ubiquitous soil fungi with strong biocontrol potential due to their ability to suppress plant pathogens. This study assessed the antifungal activity of Trichoderma harzianum, Trichoderma asperellum, Trichoderma virens, and Trichoderma lixii against Phytophthora capsici, Pyricularia oryzae, and Fusarium verticillioides using dual culture and double plate assays on Potato Dextrose Agar (PDA). Percentage inhibition of radial growth (PIRG) was calculated, and mycoparasitism mechanisms were confirmed via Scanning Electron Microscopy (SEM), showing hyphal coiling, penetration, and sporulation. In dual culture assays, T. asperellum exhibited the highest inhibition against P. capsici (85.19 ± 1.57%) and P. oryzae (79.63 ± 0.94%), while T. virens showed the most significant inhibition against F. verticillioides (72.22 ± 2.70%). In double plate assays, T. asperellum again showed potent inhibition, particularly against P. capsici (76.30 ± 1.57%) and P. oryzae (71.11 ± 0.94%), whereas T. virens remained most effective against F. verticillioides (68.52 ± 2.70%). Trichoderma lixii displayed comparatively lower inhibition across all pathogens and assays. These findings reinforce the potential of Trichoderma spp. as effective biocontrol agents and support their broader application in sustainable agriculture to reduce reliance on chemical fungicides.